| Print Close window |
| Session Information |
|---|
| Session Title: Techniques and Genomics Session Type: Poster |
| Session Location: Grand Exhibit Hall Session Time: Thursday - Saturday |
| Presentation Information |
| Poster Board Number: 775A Presentation Time: THU 3:00PM-4:00PM, 8:00PM-9:00PM; FRI 10:00PM-11:00PM; SAT 1:30PM-2:30PM; |
| Keywords: KW13e - gene and transcript mapping |
| Presentation Content |
|
Genome-Wide Expression-Based Lineage Analysis. John M. Olson1, Cory J. Evans1, Eunha Kim1, Kathy Ngo1, Noemi E. Lee1, Edward Kuoy1, Alexander N Patananan1, Daniel Sitz1, PhuongThao Tran1, Minh-Tu Do1, Kevin Yackle1, Albert Cespedes1, Gerald B. Call2, The UCLA URCFG1, Volker Hartenstein1, Utpal Banerjee1. 1) Molecular, Cell, and Developmental Biology, University of California, Los Angeles, Los Angeles, CA; 2) Midwestern University, Glendale, AZ. The UCLA Undergraduate Research Consortium for Functional Genomics has undertaken the development of a comprehensive genome based analysis of lineage in several tissues of Drosophila. We have created a Drosophila chromosome that has the following genotype: UAS-FLP, UAS-RFP, Ubiquitin promoter-FRT-STOP CASSETTE-FRT-GFP. Students assigned various Gal4 lines from the NP consortium GET DB project, cross into the above background as follows:Random enhancer-Gal4 X UAS-FLP, UAS-RFP, ubi-FRT-STOP-FRT-GFP. In the progeny, the Gal4 is expressed in a pattern that is characteristic of the enhancer. This causes expression of Flp in these cells, excision of the stop cassette and permanent marking of these cells and all their progeny with GFP under the control of the ubiquitously expressed promoter. additionally, the RFP reporter continues to monitor the expression pattern due to the enhancer in real time. By comparing the RFP and GFP patterns the students are providing a comprehensive gene-expression based lineage map for the genes in the developing brain, blood, and imaginal discs. |
| Print Close window |