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| Session Information |
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| Session Title: Genome and Chromosome Structure Session Type: Poster |
| Session Location: Grand Exhibit Hall Session Time: Thursday - Saturday |
| Presentation Information |
| Poster Board Number: 279C Presentation Time: THU 3:00PM-4:00PM, 10:00PM-11:00PM; FRI 8:00PM-9:00PM; SAT 1:30PM-2:30PM; |
| Keywords: KW03c - telomere |
| Presentation Content |
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Effect of 2L TAS deficiencies on telomere-telomere associations. Radmila Capkova Frydrychova1, Trevor Archer2, James Mason1. 1) LMG, NIEHS, RTP, NC; 2) LMC, NIEHS, RTP, NC. Drosophila telomeres consist of two DNA domains: a terminal array of retrotransposons, HTT, and a subterminal repetitive telomere associated sequence, TAS, a source of telomere position effect, TPE. Deletion of the 2L TAS leads to a suppression of TPE on telomeric white transgenes located at homologous and nonhomologous telomeres. Flies with a TAS deficiency show an increase in telomeric w transcript during development, especially in pupae. Moreover, a read-through w transcript initiated from a HeT-A promoter also increased with the presence of a 2L TAS deficiency. One explanation for this genetic interaction is physical associations between telomeres. We, therefore ask whether absence of 2L TAS leads to changes in nuclear chromosome organization, telomere-telomere associations, or associations of telomeres with nuclear lamina. A second explanation, which we are also testing, is TAS may regulate HeT-A transcription through an RNAi mechanism. |
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